Compact multi-enzyme pathways in P. pastoris
نویسندگان
چکیده
منابع مشابه
Compact multi-enzyme pathways in P. pastoris.
We report for the first time the functional simultaneous expression of nine genes from a single 2A peptide based polycistronic expression construct. The feasibility and arising opportunities for the biosynthetic pathway balancing for chemical production were demonstrated by the co-expression of the violacein and carotenoid biosynthesis pathways.
متن کاملFunctional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris.
N-glycans are synthesized in both yeast and mammals through the ordered assembly of a lipid-linked core Glc(3)Man(9)GlcNAc(2) structure that is subsequently transferred to a nascent protein in the endoplasmic reticulum. Once folded, glycoproteins are then shuttled to the Golgi, where additional but divergent processing occurs in mammals and fungi. We cloned the Pichia pastoris homolog of the AL...
متن کاملDETERMINISTIC p-COMPACT GROUPS
We investigate the class of N -determined p-compact groups and the class of p-compact groups with N -determined automorphisms. A p-compact group is said to be N -determined if it is determined up to isomorphism by the normalizer of a maximal torus. The automorphisms of a p-compact group are said to be N -determined if they are determined by their restrictions to this maximal torus normalizer.
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ژورنال
عنوان ژورنال: Chemical Communications
سال: 2015
ISSN: 1359-7345,1364-548X
DOI: 10.1039/c4cc08502g